Cell Line



BCRC Number: 60135 Add To Bookmark
Name: CA-HPV-10
Type: Human prostate adenocarcinoma, HPV-18-transfected
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 63 years
Gender: Male
Tissue: prostate; adenocarcinoma; human papillomavirus 18 (HPV-18) transfected

Morphology: Epithelial
Split Ratio: 1:3 to 1:4
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Cytogenetics: aneuploid
Karyology: Aneuploid; modal number = 72
Tumorigenic: no; The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: Keratinocyte-serum free medium (GIBCO-BRL #17005-042) with 5 ng/ml human recombinant epidermal growth factor and 50 ug/ml bovine pituitary extract
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA; stop trypsin activity by neutralization with 0.1% soybean trypsin inhibitor or centrifugation at 1000 rpm for 10 minutes ,resuspend the pellet in fresh medium, aspirate and sispense into new flasks.
Freezed Medium: 85% culture medium + 10% fetal bovine serum + 5% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from a prostatic adenocarcinoma of Gleason Grade 4/4 from a 63-year-old Caucasian male; the cells were transfected with HPV18 DNA; incorporation of HPV-18 DNA was confirmed by PCR; specific amplification of a 160-base pair fragment of the HPV-18 E6 transforming region was noted.
Others: STR-PCR profile — D7S820:10, 11 CSF1PO:10, 11 TH01:6, 8 D13S317:11, 12 D16S539:11 vWA:16, 17 TPOX:8, 9 Amelogenin:X, Y D5S818:11, 12

Image / Description:   200倍視野下
 

Biosafety Level: 2
Reference: Cancer Res. 54:5579-5583, 1994
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