Cell Line

BCRC Number: 60132 Add To Bookmark
Name: DLD-1
Type: Human colorectal adenocarcinoma
Species: Homo sapiens (human)
Age Stage: Dukes' type C
Gender: Male
Tissue: Colon; adenocarcinoma

Morphology: Epithelial
Split Ratio: 1:2 to 1:10
Mycoplasma Test: Negative; NOTE: The line has been cured of a previous mycoplasma infection at ATCC.
Karyology: Pseudodiploid
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium +with 2mM L-glutamine, 4.5 g/L glucose, 10mM HEPEs,and 1 mM sodium pyruvate, 10% FBS
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: DNA fingerprinting and cytogenetic analyses show the line is similar to HCT-15 (see BCRC 60539) and suggest the two are of different clonal origin from the same individual; their genetic origin has been confirmed by DNA fingerprinting.
Others: STR-PCR profile — D7S820:10, 12 CSF1PO:11, 12 TH01:7, 9.3 D13S317:8, 11 D16S539:12, 13 vWA:18, 19 TPOX:8, 11 Amelogenin:X, Y D5S818:13
Product Character: carcinoembryonic antigen (CEA) 0.5 ng/106 cells/10 days; colon antigen 3; keratin

Image / Description:   200倍視野下低細胞密度
  100倍視野下 高細胞密度
  200倍視野下 高細胞密度

Biosafety Level: 1
Reference: Cancer Res. 39: 1020-1025, 1979; Cancer Genet. Cytogenet. 10: 351-362, 1983; Cancer Genet. Cytogenet. 81: 103-108, 1995; Int. J. Cancer 41: 287-296, 1988; Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990
Frozen Vial for all NTD$ 6000 Order this item

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