Cell Line

BCRC Number: 60125 Add To Bookmark
Name: NCI-H661
Type: Human lung large cell carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 43 years
Gender: Male
Tissue: Lung; large cell carcinoma; from metastatic site: lymph node

Morphology: Epithelial
Split Ratio: 1:3 to 1:5
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Cytogenetics: hyperhexaploid; modal number = 142; range = 130 to 153
Karyology: Hyperhexaploid; modal number = 142

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium + 10% fetal bovine serum (if desired, the serum can be reduced to 5%)
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: derived from the lymph node of a patient with large cell cancer of the lung; the specimen was obtained prior to therapy; the line lacks ultrastructural and biochemical evidence of squamous differentiation or mucin production; the cells express easily dete
Others: STR-PCR profile — D7S820:8, 10 CSF1PO:10 TH01:8 D13S317:11 D16S539:12 vWA:17 TPOX:8 Amelogenin:X, Y D5S818:11

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: Cancer Res. 50: 120-128, 1990; Science 246: 491-494, 1989; Cancer res. 45: 1187-1197, 1985
Frozen Vial for all NTD$ 6000 Order this item

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