Cell Line

BCRC Number: 60124
Name: NCI-H520
Type: Human lung squamous cell carcinoma
Species: Homo sapiens (human)
Gender: Male
Tissue: Lung; squamous cell carcinoma

Morphology: Epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Cytogenetics: hyperdiploid
Karyology: Hypotriploid; modal number = 58
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: derived from a sample of a lung mass taken from a patient with squamous cell carcinoma of the lung; the cells express greatly reduced levels of p53 mRNA relative to normal lung tissue, but exhibit no gross structural DNA abnormalities; the cells stain pos
Others: STR-PCR profile — D7S820:8, 12 CSF1PO:10 TH01:10 D13S317:10, 11 D16S539:8, 13 vWA:18, 19 TPOX:8 Amelogenin:X D5S818:12, 13

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: Science 246: 491-494, 1989; Cancer Res. 45: 1187-1197, 1985; Cancer Res. 46: 798-806, 1986
Frozen Vial for all NTD$ 6000 Order this item

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

web maintainance: +886-3-5223191 ext 593

Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).