Cell Line



BCRC Number: 60124
Name: NCI-H520
Type: Human lung squamous cell carcinoma
Species: Homo sapiens (human)
Gender: Male
Tissue: Lung; squamous cell carcinoma

Morphology: Epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Cytogenetics: hyperdiploid
Karyology: Hypotriploid; modal number = 58
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: derived from a sample of a lung mass taken from a patient with squamous cell carcinoma of the lung; the cells express greatly reduced levels of p53 mRNA relative to normal lung tissue, but exhibit no gross structural DNA abnormalities; the cells stain pos
Others: STR-PCR profile — D7S820:8, 12 CSF1PO:10 TH01:10 D13S317:10, 11 D16S539:8, 13 vWA:18, 19 TPOX:8 Amelogenin:X D5S818:12, 13

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Science 246: 491-494, 1989; Cancer Res. 45: 1187-1197, 1985; Cancer Res. 46: 798-806, 1986
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

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