Cell Line



BCRC Number: 60123
Name: NCI-H209
Type: Human lung small cell carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Gender: Male
Tissue: Lung; small cell carcinoma; from metastatic site: bone marrow

Morphology: Round cell clusters
Split Ratio: 1:2 to 1:3
Mycoplasma Test: Negative
Cytogenetics: hyperdiploid
Karyology: Hyperdiploid; modal number = 49
Tumorigenic: yes; forms transplantable tumors with typical SCLC histology in nude mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Iscove's modified Dulbecco's medium + 10% fetal bovine serum
Growth Property: Aggregates in suspension; only the aggregates are viable, but no meaningful viability percentage can be measured; the medium will normally contain large amounts of cell debris
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: derived from the bone marrow of a patient with small cell cancer of the lung; the bone marrow specimen was taken prior to therapy; the line is a classic SCLC cell line which expresses elevated levels of four biochemical markers (neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bomesin-like immunoreactivity.
Others: STR-PCR profile — D7S820:9 CSF1PO:11 TH01:7, 9 D13S317:11 D16S539:9, 12 vWA:18, 19 TPOX:8 Amelogenin:X,Y D5S818:12

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Cancer Res. 45: 2913-2923, 1985, Science 246: 491-494, 1989; Nature 306: 194-196, 1983
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