Cell Line



BCRC Number: 60122
Name: PC-3
Type: Human prostate carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 62 years; grade IV
Gender: Male
Tissue: Prostate; adenocarcinoma; from metastatic site: bone

Morphology: Epithelial
Split Ratio: 1:3 to 1:6
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Karyology: Near-triploid; modal number = 62
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 93% Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate + 7% fetal bovine serum
Growth Property: monolayer; the cells form clusters in soft agar and can be adapted to suspension growth
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the line was initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian; the cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities.
Others: STR-PCR profile — D7S820:8, 11 CSF1PO:11 TH01:6, 7 D13S317:11 D16S539:11 vWA:17 TPOX:8, 9 Amelogenin:X D5S818:13

Image / Description:   100倍視野下高密度細胞照片
  200倍視野下高密度細胞照片
 

Biosafety Level: 1
Reference: Invest. Urol. 17: 16-23, 1979; Cancer Res. 40: 524-534, 1980
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