Cell Line



BCRC Number: 60120
Name: Hs 578T
Type: Human breast ductal carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 74 years
Gender: Female
Tissue: Breast; mammary gland; ductal carcinoma

Morphology: Epithelial
Split Ratio: 1:2 to 1:3
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Karyology: Hypotriploid
Tumorigenic: no; The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 10 μg/ml bovine insulin + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the line was derived from a carcinoma of the breast; it was originated along with the Hs 578Bst, which is a normal fibroblast-like line from the same patient; the line had a mixed polygonal morphology initially, but a stellate cell type was selected for during passage and by cioning,
Others: STR-PCR profile — D7S820:10 CSF1PO:13 TH01:9, 9.3 D13S317:11 D16S539:9, 12 vWA:17 TPOX:8 Amelogenin:X D5S818:11

Image / Description:   200倍視野下
  100倍視野下高細胞密度
  200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 58: 1795-1806, 1977
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