Cell Line



BCRC Number: 60119
Name: Detroit 562
Type: Human pharynx carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: Adult
Gender: Female
Tissue: Pharynx; carcinoma; from metastatic site: pleural effusion

Morphology: Epithelial
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: negative
Karyology: Heteroploid
Virus susceptibility: vesicular stomatitis (Indiana); poliovirus 1

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 0.1% lactalbumin hydrolysate+ 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from metastatic carcinomatous cells in pleural fluid from an adult Caucasian female who had a primary carcinoma of the pharynx; the cells are positive for keratin by immunoperoxidase staining.
Others: STR-PCR profile — D7S820:8, 10 CSF1PO:11, 13 TH01:8, 9 D13S317:12 D16S539:11 vWA:16 TPOX:8, 10 Amelogenin:X D5S818:11, 12
Product Character: keratin

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Proc. Soc. Exp. Biol. Med. 128: 772-776, 1968; Proc. Soc. Exp. Biol. Med. 136: 1187-1191, 1971
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