Cell Line



BCRC Number: 60119
Name: Detroit 562
Type: Human pharynx carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: Adult
Gender: Female
Tissue: Pharynx; carcinoma; from metastatic site: pleural effusion

Morphology: Epithelial
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: negative
Karyology: Heteroploid
Virus susceptibility: vesicular stomatitis (Indiana); poliovirus 1

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 0.1% lactalbumin hydrolysate+ 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from metastatic carcinomatous cells in pleural fluid from an adult Caucasian female who had a primary carcinoma of the pharynx; the cells are positive for keratin by immunoperoxidase staining.
Others: STR-PCR profile — D7S820:8, 10 CSF1PO:11, 13 TH01:8, 9 D13S317:12 D16S539:11 vWA:16 TPOX:8, 10 Amelogenin:X D5S818:11, 12
Product Character: keratin

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Proc. Soc. Exp. Biol. Med. 128: 772-776, 1968; Proc. Soc. Exp. Biol. Med. 136: 1187-1191, 1971
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

web maintainance: +886-3-5223191 ext 593

Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).