Cell Line



BCRC Number: 60117 Add To Bookmark
Name: HEL 299
Type: Human embryonic lung
Species: Homo sapiens (human)
Strain Ethnicity: Black
Age Stage: fetus
Gender: Male
Tissue: Lung; normal; fibroblast

Morphology: Fibroblast
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Cytogenetics: diploid; normal human male; stable
Karyology: Normal human male; diploid; stable
Virus susceptibility: vesicular stomatitis (Indiana); poliovirus 1
Receptor expressed: acetylcholine, muscarinic m2

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 0.1% lactalbumin hydrolysate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: the capacity of this cell line to propagate in culture is limited, and they will eventually senesce; cells from the current distribution stocks are capable of 15-18 population doublings before the onset of senescence; M2 muscarinic receptor expression is downregulated following protein kinase C stimulation.
Others: STR-PCR profile — D7S820:8, 11 CSF1PO:7, 10 TH01:7 D13S317:11, 12 D16S539:10, 11 vWA:16 TPOX:8, 12 Amelogenin:X, Y D5S818:11, 13

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Proc. Soc. Exp. Biol. Med. 128: 772-776, 1968; . Biol. Chem. 270: 7213-7218, 1995
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