Cell Line

BCRC Number: 60116 Add To Bookmark
Name: Raji
Type: Human Burkitt's lymphoma
Species: Homo sapiens (human)
Strain Ethnicity: Black
Age Stage: 11 years
Gender: Male
Tissue: lymphoma; Burkitt's lymphoma; B lymphocyte

Morphology: Lymphoblast
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Male; diploid; stable
Virus resistance: The cells are partially resistant to poliovirus and vesicular stomatitis viruses.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 4 X 10 5 viable cells/ml; A maximum of 3 x 10 6 viable cells/ml is obtainable.
Medium Renewal: every 2 to 3 days

Description: Derived from Burkitt's lymphoma of the left maxilla of an 11-year-old Black male; the cells are EBNA positive
Others: STR-PCR profile — D7S820:10 CSF1PO:10, 12 TH01:6, 7 D13S317:13 D16S539:8, 11 vWA:16, 19 TPOX:8, 13 Amelogenin:X, Y D5S818:10, 13

Image / Description:   200倍視野下

Biosafety Level: 2
Reference: Lancet 1: 238-240, 1964; J. Natl. Cancer Inst. 34: 231-240, 1965; J. Natl. Cancer Inst. 37: 547-559, 1966; Trans. N.Y. Acad. Sci. 29: 61, 1966
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