Cell Line



BCRC Number: 60115 Add To Bookmark
Name: IM-9
Type: Human multiple myeloma leukemia, immunoglobulin secreting
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Gender: Female
Tissue: peripheral blood; B lymphoblast; Epstein-Barr virus (EBV) transformed

Morphology: Lymphoblast
Split Ratio: maintain cell density between 105 to 106 cells/ml
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Cytogenetics: diploid; normal human female; stable
Karyology: Normal human female; diploid; stable
Receptor expressed: growth hormone; insulin; calcitonin

Incubation Temp(°C): 37°C
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Growth Property: suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 1-2 x 105 cells/ml
Medium Renewal: every 2 to 3 days

Description: although derived from the blood of a patient with multiple myeloma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line; the cells are EBNA positive
Others: STR-PCR profile — D7S820:11, 12 CSF1PO:10, 11 TH01:6, 9.3 D13S317:9, 11 D16S539:9, 13 vWA:14, 17 TPOX:11 Amelogenin:X D5S818:13
Product Character: immunoglobulin

Image / Description:   100X 視野下
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Biosafety Level: 2
Reference: Ann. N.Y. Acad. Sci. 190: 221-234, 1972; Nature 251: 443-444, 1974; Proc. Natl. Acad. Sci. USA 71: 84-88, 1974; J. Biol. Chem. 249: 1661-1667, 1974; J.B.C., 251: 6844-6851, 1976
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