Cell Line



BCRC Number: 60114
Name: Clone C6/36 (Aedes albopictus Clone C6/36)
Type: Mosquito larvae
Species: Aedes albopictus (mosquito)
Age Stage: Larva
Gender: unknown
Tissue: Larva, whole

Split Ratio: 1:4 to 1:10
Mycoplasma Test: Negative
Cytogenetics: diploid
Karyology: Diploid
Virus susceptibility: flaviviruses (dengue viruses)
Tumorigenic: no; The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.

Incubation Temp(°C): 28°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium Eagle with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: by scraping or vigorous pipetting
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 3 to 4 days

Description: derived from the Aedes albopictus cell line; the cells are not anchorage dependent; this cell line is useful for the replication of flaviviruses and can be used to replicate Denque viruses to high titers .

Image / Description:   100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: J. Gen. Virol. 40: 531-544, 1978; Curr. Sci. 36: 506-508, 1967
Shipping/Field/Price:
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