Cell Line



BCRC Number: 60113 Add To Bookmark
Name: RD
Type: Human embryonal rhabdomyosarcoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 7 years
Gender: Female
Tissue: muscle; rhabdomyosarcoma

Morphology: Spindle cells and large multinucleated cells
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Karyology: Unstable hyperdiploid; modal number = 49 and 50
Virus susceptibility: poliovirus 1; vesicular stomatitis (Indiana); herpes simplex; vaccinia

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from a malignant embryonal rhabdomyosarcoma of the pelvis of a 7-year-old Caucasian female; the line has also been designed as TE32 and 130T in the literature; the cells contain myoglobin and myosin-ATPase activity; this line has recently been sho
Others: STR-PCR profile — D7S820:8, 12 CSF1PO:10, 11 TH01:9.3 D13S317:13 D16S539:10, 11 vWA:18 TPOX:9 Amelogenin:X D5S818:11
Product Character: myoglobin; myosin ATPase

Image / Description:   100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: Cancer 24: 520-526, 1969; J. Virol. 1: 326, 1967
Shipping/Field/Price:
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