Cell Line

BCRC Number: 60111
Name: RT4
Type: Human bladder carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 63 years
Gender: Male
Tissue: urinary bladder; transitional cell papilloma

Morphology: Epithelial
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Karyology: Aneuploid
Tumorigenic: yes, in cheek pouch of steroid treated hamsters

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% McCoy's 5a medium + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from explants of a recurring papillary tumor of the bladder; a contamination with Mycoplasma orale was cured in October 1971
Others: STR-PCR profile — D7S820:9, 12 CSF1PO:10, 12 TH01:9, 9.3 D13S317:8 D16S539:9 vWA:14, 17 TPOX:8, 11 Amelogenin:X, Y D5S818:11, 12

Image / Description:   200倍視野下

Biosafety Level: 1
Reference: Br. J. Cancer 24: 746-754, 1970; Int. J. Cancer. 10: 77-91, 1972; In vitro models for cancer research, Vol. IV. CRC Press: pp.103-125, 1986
Frozen Vial for all NTD$ 6000 Order this item

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

web maintainance: +886-3-5223191 ext 593

Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).