Cell Line



BCRC Number: 60111
Name: RT4
Type: Human bladder carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 63 years
Gender: Male
Tissue: urinary bladder; transitional cell papilloma

Morphology: Epithelial
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Karyology: Aneuploid
Tumorigenic: yes, in cheek pouch of steroid treated hamsters

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% McCoy's 5a medium + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from explants of a recurring papillary tumor of the bladder; a contamination with Mycoplasma orale was cured in October 1971
Others: STR-PCR profile — D7S820:9, 12 CSF1PO:10, 12 TH01:9, 9.3 D13S317:8 D16S539:9 vWA:14, 17 TPOX:8, 11 Amelogenin:X, Y D5S818:11, 12

Image / Description:   200倍視野下
  100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: Br. J. Cancer 24: 746-754, 1970; Int. J. Cancer. 10: 77-91, 1972; In vitro models for cancer research, Vol. IV. CRC Press: pp.103-125, 1986
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

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