Cell Line



BCRC Number: 60109 Add To Bookmark
Name: COLO 320 HSR
Type: Human colon adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 55 years
Gender: Female
Tissue: Colon; adenocarcinoma

Morphology: Rounded and refractile
Split Ratio: 1:3 to 1:4
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Cytogenetics: marker chromosomes with homogeneously staining regions (HSR) were observed in the double minute (DM) chromosomes appeared in much lower frequency than the parental line COLO 320DM
Karyology: Hyperdiploid; marker chromosomes with homogeneously staining regions (HSR) were observed and double minutes were in the lower frequency than the parent line COLO 320DM (CCRC 60108)
Tumorigenic: yes, Tumors developed within 21 days at 80% frequency (4/5) in nude mice inoculated subcutaneously with 10(7) cells.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI medium 1640 medium + 10% fetal bovine serum
Growth Property: Loosely attached and in suspension
SubCulture Procedure: shake flask or use trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: This line is a subline of COLO 320DM (see BCRC 60108) developed after 16 months of continuous subculture; negative for CEA and CSAp; positive for Colon antigen 3; the cells are weakly positive for keratins and vimentin
Others: STR-PCR profile — D7S820:9,12 CSF1PO:11 TH01:8,9 D13S317:11 D16S539:11,12 vWA:15, 18 TPOX:8, 9 Amelogenin:X D5S818:12
Product Character: serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone

Image / Description:   200倍視野下
 

Biosafety Level: 1
Reference: Cancer Res. 39:4914-4924, 1979; Int. J. Cancer 41: 287-296, 1988
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