Cell Line

BCRC Number: 60108
Name: COLO 320DM
Type: Human colon adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 55 years; Dukes' type C
Gender: Female
Tissue: Colon; adenocarcinoma

Morphology: Rounded and refractile
Split Ratio: 1:5 to 1:10
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Cytogenetics: double minutes (DM) were observed in 24% of the metaphases examined
Karyology: Hyperdiploid with 9-11 marker chromosomes; double minutes (DM) were found
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (4/4) in nude mice inoculated subcutaneously with 10(7) cells.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI medium 1640 + 10% heat-inactivated fetal bovine serum
Growth Property: Loosely attached and in suspension
SubCulture Procedure: shake flask or use trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 4 to 7 days

Description: Derived from a moderately undifferentiated adenocarcinoma of the sigmoid colon from a 55-year-old female Caucasian; initial cytogenetic preparations revealed numerous double minute (DM) chromosomes, a prominent characteristic of this colon carcinoma.
Others: STR-PCR profile — D7S820:9,12 CSF1PO:11 TH01:9 D13S317:11 D16S539:11,12 vWA:15, 18 TPOX:8, 9 Amelogenin:X D5S818:12
Product Character: serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone

Image / Description:   100倍視野下細胞形態

Biosafety Level: 1
Reference: Cancer Res. 39:4914-4924, 1979
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