Cell Line

BCRC Number: 60106
Name: MOLT-3
Type: Human acute lymphoblastic leukemia, T cell
Species: Homo sapiens (human)
Age Stage: 19 years
Gender: Male
Tissue: leukemia; acute lymphoblastic; T lymphoblast

Morphology: Lymphoblast
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Hypertetraploid; modal number = 97

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI medium 1640 + 10% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 2-4 x 10 5 cells/ml
Medium Renewal: add fresh medium every 3 to 4 days

Description: established from cells taken from a patient in relapse; the patient had received prior multidrug chemotherapy; no immunoglobulin or Epstein-Barr virus is detectable; the line was derived from the same patient as the MOLT-4 cell line.
Others: STR-PCR profile — D7S820:8, 10 CSF1PO:11, 12 TH01:6, 8 D13S317:12, 13 D16S539:11, 14 vWA:17 TPOX:8 Amelogenin:X, Y D5S818:12
Product Character: The terminal deoxynucleotidyl transferase (TdT) activity is high for this cell line.

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Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 49: 891-895, 1972. Blut 43: 99-103, 1981
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