Cell Line



BCRC Number: 60105 Add To Bookmark
Name: BF-2
Type: bluegill caudal trunk
Species: Lepomis macrochirus (bluegill)
Age Stage: 1 year
Gender: unknown
Tissue: Caudal trunk

Morphology: Fibroblast
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Hyperdiploid
Virus resistance: poliovirus 1
Virus susceptibility: infectious pancreatic necrosis

Incubation Temp(°C): 23°C
Incubation Condition: 5% CO2; The cells will grow at temperature from 15°C through 33°C.
Culture Medium: 90% Minimum essential medium (Eagle) in Earle's BSS + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: at the time of subcultivation

Description: Derived from a trypsinized suspension of a pooled caudal portion of the trunk of one-year old fingerlings; it is susceptible to lymphocystis virus of fish and tadpole edema virus (TEV) of frogs.

Image / Description:   100倍視野下高細胞密度
  200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: Science 151: 1004-1005, 1966; J. Virol. 2: 393-399, 1968; J. Infect. Dis. 118: 253-262, 1968
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