Cell Line



BCRC Number: 60102 Add To Bookmark
Name: AGS
Type: Human gastric adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 54 years
Gender: Female
Tissue: Stomach; adenocarcinoma

Morphology: Epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative; NOTE: The line has been cured of a previous mycoplasma infection.
Karyology: Hyperdiploid; modal number = 49
Tumorigenic: yes, in athymic BALB/c mice
Doubling time: 20 hrs

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Ham's F-12K medium + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from an adenocarcinoma of the stomach resected from a 54-year-old Caucasian female who had received no prior anticancer therapy. AGS are persistently infected with parainfluenza virus type 5(PIV5;previously known as SV5).
Others: STR-PCR profile — D7S820:10, 11 CSF1PO:11, 12 TH01:6, 7 D13S317:12 D16S539:11, 13 vWA:16, 17 TPOX:11, 12 Amelogenin:X D5S818:9, 12

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Biosafety Level: 2
Reference: Cancer Res. 43:1703-1709, 1983; Invest. New Drugs 1:117-127,1983
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