Cell Line



BCRC Number: 60100 Add To Bookmark
Name: LS 180
Type: Human colon adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 58 years; Dukes' type B
Gender: Female
Tissue: Colon; adenocarcinoma

Morphology: Epithelial
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: G6PD, B; MD; LD; NP
Mycoplasma Test: Negative
Karyology: Stemline number = 45
Receptor expressed: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium (Eagle) with Earle's BSS , 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: by scraping; NOTE: The cells have never been trypsinized.
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: LS 180 and LS 174T (see BCRC 60053) were both derived from the same patient, a 58-year-old female with Dukes' type B adenocarcinoma of the colon; LS 180 has never been trypsinized, however the variant LS 174T has been subcultured by trypsinization.
Others: STR-PCR profile — D7S820:11 CSF1PO:10, 13 TH01:5.3, 7 D13S317:10 D16S539:10, 11, 13 vWA:15, 18 TPOX:8, 9 Amelogenin:X D5S818:10, 15
Product Character: carcinoembryonic antigen (CEA) 1916 ng/106 cells in 10 days; mucin; interleukin 10 (IL-10, interleukin-10); interleukin 6 (IL-6, interleukin-6)

Image / Description:   200倍視野下低細胞密度
 

Biosafety Level: 1
Patent Statement: U.S. Pat. 4,228,236
Reference: In Vitro 12: 180-191, 1976; U.S. Pat. 4,228,236 dated Oct. 14, 1980
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