Cell Line

BCRC Number: 60092 Add To Bookmark
Name: LLC-MK2
Type: Rhesus monkey kidney
Species: Macaca mulatta (monkey, Rhesus)
Age Stage: Adult
Gender: unknown
Tissue: Kidney; normal

Morphology: Epithelial
Split Ratio: 1:2 to 1:10
Isoenzyme Analysis: MD; G6PD; LD; NP
Mycoplasma Test: Negative
Virus susceptibility: poliovirus 1; coxsackievirus B4; bovine viral diarrhea (BVD) virus

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 99% Medium 199 (with 1.68 g/L bicarbonate) + 1% horse serum; Note: The serum should be pre-tested for good quality. Some lots of horse serum do not give satisfactory growth. Adaptation to other sera generally results in slow growth with a prolonged lag.
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from a pooled cell suspension from kidneys removed from six adult Rhesus monkeys; the line is susceptible to a broad range of viruses that includes members of the enterovirus, rhinovirus, myxovirus, and poxvirus groups
Product Character: plasminogen activator

Image / Description:   200倍視野下高細胞密度

Biosafety Level: 1
Reference: Anat. Rec. 124: 490, 1956; Natl. Cancer Inst. Monogr. 7: 161, 1962; J. Exp. Med. 115: 903, 1962
Frozen Vial for all NTD$ 6000 Order this item

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