Cell Line

BCRC Number: 60083 Add To Bookmark
Name: C2C12
Type: Mouse muscle myoblast
Species: Mus musculus (mouse)
Strain Ethnicity: C3H
Age Stage: Adult
Gender: unknown
Tissue: Muscle; myoblast

Morphology: fibroblast
Isoenzyme Analysis: MD; G6PD; LD
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA; NOTE: Do not allow the culture to reach confluency as this will deplete the myoblastic population in the culture.
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: between 1.5 X 105 and 1.0 X 10 6 viable cells/75 cm2
Medium Renewal: every 2 to 3 days

Description: this is a subclone derived from the mouse myoblast cell line established from normal adult C3H mouse leg muscle; the cells differentiate rapidly and produce extensive contracting myotubes expressing characteristic muscle proteins; treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.

Image / Description:   200倍視野下低細胞密度

Biosafety Level: 1
Reference: Nature 270: 725-727, 1977; Science 230:758-766, 1985
Frozen Vial for all NTD$ 6000 Order this item

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