Cell Line



BCRC Number: 60074
Name: A-549
Type: Human lung carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 58 years
Gender: Male
Tissue: Lung; carcinoma

Morphology: Epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: MD; G6PD; LD; NP
Mycoplasma Test: Negative
Karyology: Hypotriploid

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: This line was initiated through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male; the cells could synthesize lecithin with a high percentage of desaturated fatty acids utilizing the cytidine diphosphocholine pathway.
Others: STR-PCR profile — D7S820:8, 11 CSF1PO:10, 12 TH01:8, 9.3 D13S317:11 D16S539:11, 12 vWA:14 TPOX:8, 11 Amelogenin:X, Y D5S818:11

Image / Description:   100倍視野下
  200倍視野下
  100倍視野下 高細胞密度
  200倍視野下 高細胞密度
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst 51: 1417-1423, 1973; Int. J. Cancer 17: 62-70, 1976
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