Cell Line



BCRC Number: 60073 Add To Bookmark
Name: BeWo
Type: Human choriocarcinoma, secreting gonadotropin, lactogen and steroid
Species: Homo sapiens (human)
Gender: male
Tissue: Placenta; choriocarcinoma

Morphology: Epithelial
Split Ratio: 1:3
Isoenzyme Analysis: MD; G6PD, B; LD; NP
Mycoplasma Test: Negative
Cytogenetics: hypotetraploid
Karyology: Hypotetraploid
Virus susceptibility: poliovirus 3; vesicular stomatitis (Indiana)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 85% Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate + 15% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: 3 to 4 times per week(The predominant glycolytic metabolism exhibited by these cells and a high carbon dioxide production and rapid drop in PH, necessitates daily fluid renewals or addition of sufficient culture medium to last 36-48 hours to prevent glucose exhaustion. )

Others: STR-PCR profile — D7S820:10, 12 CSF1PO:11, 12 TH01:9, 9.3 D13S317:9, 11 D16S539:13, 14 vWA:16 TPOX:8 Amelogenin:X, Y D5S818:10, 11
Product Character: progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); estrogen; estrone; estriol; estradiol; keratin

Image / Description:   200倍視野下高細胞密度
  200倍視野下低細胞密度
  100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: Cancer Res. 28: 1231, 1968; Science 159: 1467, 1968; Proc. Soc. Exp. Biol. Med. 102: 77, 1959; In Vitro (Rockville) 6: 205, 1970; Ann. N. Y. Acad. Sci. 172:288, 1971; J. Clin. Endocrinol. Metab. 34: 59-61, 1972
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