Cell Line



BCRC Number: 60071
Name: 3T3
Type: Mouse embryonic fibroblast, contact-inhibited
Species: Mus musculus (mouse)
Strain Ethnicity: 3T3-Swiss albino
Age Stage: Embryo
Gender: unknown
Tissue: Embryo; fibroblast

Morphology: Fibroblast
Isoenzyme Analysis: MD; LD; G6PD; NP
Mycoplasma Test: Negative
Cytogenetics: hypertriploid
Karyology: Hypertriploid; modal chromosome number = 68
Virus susceptibility: polyomavirus; SV40
Doubling time: 18hours

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% bovine calf serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA; NOTE: Never allow cultures to become completely confluent.
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: For plates (50mm) use an inoculum of 3 X 105 cells per plate and subculture every 3 days. For 75 sq cm flasks use 4 X 105 per flask and subculture every 3 days.
Medium Renewal: every 2 to 3 days

Description: established from disaggregated Swiss mouse embryos; the cells are contact inhibited; a confluent monolayer yields 40000 cells/sq cm; a saturation density of approximately 50000 cells per sq cm can be reached.

Image / Description:   200被視野下
 

Biosafety Level: 1
Reference: J. Cell Biol. 17: 299-313, 1963; Virology 16: 41-51, 1962; ibid. 27: 179-185, 1965; Proc. Natl. Acad. Sci. USA 51: 66-73, 1964
Shipping/Field/Price:
Frozen Vial for all NTD$ 6000 Order this item

Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

Copyright © 2018.BCRC All rights reserved.

web maintainance: +886-3-5223191 ext 593