Cell Line



BCRC Number: 60070
Name: 3T6
Type: Mouse embryo, collagen-secreting
Species: Mus musculus (mouse)
Strain Ethnicity: 3T6-Swiss albino
Age Stage: Embryo
Gender: unknown
Tissue: Embryo; fibroblast

Morphology: Fibroblast
Split Ratio: 1:4 to 1:10
Isoenzyme Analysis: G6PD, B; MD; LD
Mycoplasma Test: Negative
Karyology: Heteroploid; stemline number = 78 to 81
Virus resistance: poliovirus 2
Virus susceptibility: herpes simplex; vaccinia; pseudorabies; vesicular stomatitis (Indiana)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: this is a collagen and hyaluronic acid secreting line established from disaggregated Swiss mouse embryos .
Product Character: collagen; hyaluronic acid

Image / Description:   200倍視野下高細胞密度
  100倍視野下高細胞密度
 

Biosafety Level: 1
Reference: J. cell Biol. 17: 299-313, 1963
Shipping/Field/Price:
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