Cell Line



BCRC Number: 60069 Add To Bookmark
Name: BFTC 909
Type: Human renal pelvis transitional cell carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Asian (Chinese)
Age Stage: 64 years; grade 3
Gender: Male
Tissue: Kidney; renal pelvis; transitional cell carcinoma; sarcomatoid component

Morphology: Fibroblast
Split Ratio: 1:4 to 1:8
Isoenzyme Analysis: G6PD; MD; LD
Mycoplasma Test: negative
Karyology: Aneuploid

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose+ 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Deposit History: Obtained from Dr. Chien-Cherng Tzeng, National Cheng Kung University, Tainan, Taiwan.
Description: Derived from primary transitional cell carcinomas of a patient from a blackfoot disease endemic area in Taiwan; the line is tumorigenic in nude mice; no point mutation at codons 12, 13 and 61 of H-, K- and N-ras; positive for vimentin
Others: STR-PCR profile — D7S820:12 CSF1PO:12, 13 TH01:7 D13S317:11, 13 D16S539:9, 10 vWA:14, 18 TPOX:8 Amelogenin:X D5S818:11, 12

Image / Description:   400倍視野下高細胞密度
  100倍視野下
  200倍視野下
 

Biosafety Level: 1
Reference: Anticancer Res. 16:1797-1804, 1996
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