Cell Line



BCRC Number: 60068
Name: BFTC 905
Type: Human bladder papillary transitional cell carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Asian (Chinese)
Age Stage: 51 years; grade 3
Gender: Female
Tissue: urinary bladder; papillary transitional cell carcinoma

Morphology: Epithelial
Split Ratio: 1:2 to 1:10
Isoenzyme Analysis: G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Aneuploid

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 85% RPMI 1640 medium contain 1.5 g/L sodium bicarbonate + 15% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA (very sensutive to trypsin)
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Deposit History: Obtained from Dr. Chien-Cherng Tzeng, National Cheng Kung University, Tainan, Taiwan
Description: Derived from primary transitional cell carcinomas of a patient from a blackfoot disease endemic area in Taiwan; the line is a classical TCC cell line with an unusual HSR at 11q13; it is tumorigenic in nude mice
Others: STR-PCR profile — D7S820:9, 12 CSF1PO:12 TH01:7, 8 D13S317:11 D16S539:12 vWA:14, 17 TPOX:8, 9 Amelogenin:X D5S818:13

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Biosafety Level: 1
Reference: Anticancer Res. 16:1797-1804, 1996
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