Cell Line

BCRC Number: 60067 Add To Bookmark
Name: ES-2
Type: Human ovarian carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Black
Age Stage: 47 years
Gender: Female
Tissue: Ovary; clear cell carcinoma

Morphology: Fibroblast
Split Ratio: 1:4 to 1:8
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Complex hyperdiploid
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% McCoy's 5a medium with 1.5 mM L-glutamine+ 10% fetal bovine serum (Newborn calf serum may also be used)
Growth Property: Adherent
SubCulture Procedure: Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCl, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 ml double distilled water) to dislodge cells
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: established from a surgical tumor specimen taken from a 47-year-old black woman; the tumor was described as a poorly differentiated ovarian clear cell carcinoma; the cells exhibit low to moderate resistance to a number of chemotherapeutic agents including doxorubicin, cisplatin, carmustine, etoposide and cyanomorpholinodoxorubicin (MRA-CN).
Others: STR-PCR profile — D7S820:11 CSF1PO:10, 15 TH01:9.3 D13S317:11 D16S539:11, 13 vWA:16, 17 TPOX:8, 12 Amelogenin:X D5S818:11, 13
Product Character: P glycoprotein

Image / Description:   200倍視野下高細胞密度

Biosafety Level: 1
Reference: Cancer Res. 51: 5181-5187, 1991
Frozen Vial for all NTD$ 6000 Order this item

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