Cell Line

BCRC Number: 60065 Add To Bookmark
Name: CV-1
Type: African green monkey kidney
Species: Cercopithecus aethiops (monkey, African green)
Age Stage: 141 days
Gender: Male
Tissue: Kidney; normal

Morphology: Epithelial
Split Ratio: 1:2 to 1:3
Isoenzyme Analysis: G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Pseudodiploid; modal chromosome number = 60
Virus susceptibility: poliovirus 1; herpes simplex; Eastern equine encephalitis; Western equine encephalitis; California encephalitis; SV40

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium (Eagle) with Earle's BSS , 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from the kidney of a male adult African green monkey; the line is a suitable host for transfection, especially SV 40 vectors.

Image / Description:   200倍視野下高細胞密度

Biosafety Level: 1
Reference: Proc. Natl. Acad. Sci. USA 53: 53, 1964; Virology 27: 453-457, 1965
Frozen Vial for all NTD$ 6000 Order this item

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