Cell Line

BCRC Number: 60063
Name: PA-1
Type: Human ovarian teratocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 12 years
Gender: Female
Tissue: Ovary; teratocarcinoma; from metastatic site: ascites

Morphology: Epithelial
Split Ratio: 1:4 to 1:10
Isoenzyme Analysis: G6PD, B; MD; LD
Mycoplasma Test: Negative
Karyology: Pseudodiploid; modal chromosome number = 46
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium (Eagle) with Earle's BSS , 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% heat-inactivated fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Established by culturing ascitic fluid cells from a 12-year-old Caucasian female with teratocarcinoma of the ovary; the cells form tightly knit colonies, and differentiate to form embryoid bodies when cultured in low serum concentration, or at low plating densities or when treated with 5-bromo-2'-deoxyuridine.
Others: STR-PCR profile — D7S820:9 CSF1PO:9, 12 TH01:7, 9 D13S317:9, 10 D16S539:9, 12 vWA:15, 17 TPOX:11 Amelogenin:X D5S818:11

Image / Description:   200倍視野下高細胞密度

Biosafety Level: 1
Reference: In Vitro 10: 382, 1974; J. Natl. Cancer Inst. 52: 921-930, 1974; Int. J. Cancer 25:19-32, 1980; Science 225: 643-645, 1984; Cancer Res. 54: 2568-2572, 1994
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