Cell Line

BCRC Number: 60062
Name: T24
Type: Human bladder carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 81 years
Gender: Female
Tissue: urinary bladder; transitional cell carcinoma

Morphology: Epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: MD; G6PD, B; LD
Mycoplasma Test: Negative
Tumorigenic: yes, in hamster cheek pouch; not in nude mice
Doubling time: 19 hours

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% McCoy's 5a medium with 1.5 mM L-glutamine + 10% fetal bovine serum
Growth Property: Adherent;
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Developed from a primary tumor; leukocytes and serum from patients with transitional cell carcinoma were cytotoxic to T24 and related lines; the cells contains the ras (H-ras) oncogene.
Others: STR-PCR profile — D7S820:10, 11 CSF1PO:10, 12 TH01:6 D13S317:12 D16S539:9 vWA:17 TPOX:8, 11 Amelogenin:X D5S818:10, 12
Product Character: tumor specific antigen

Image / Description:   200倍視野下高細胞密度

Biosafety Level: 1
Reference: Int. J. Cancer 5: 310-31, 1970; Int. J. Cancer 10: 77-91, 1972; Int. J. Cancer 11: 765-773, 1973; Pro. Natl. Acad. Sci. USA 73: 3278-3282, 1976; J. Natl. Cancer Inst. 58: 209-214, 1977; J. Natl. Cancer Inst. Monogr. 49: 5-9, 1978; J. Natl. Cancer Inst. 66
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