Cell Line



BCRC Number: 60061 Add To Bookmark
Name: 5637
Type: human bladder primary carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 68 years
Gender: Male
Tissue: urinary bladder; carcinoma

Morphology: epithelial
Split Ratio: 1:4 to 1:8
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: negative
Tumorigenic: yes, in nude mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate+ 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:10, 11 CSF1PO:11 TH01:7, 9 D13S317:11 D16S539:9 vWA:16, 18 TPOX:8, 9 Amelogenin:X, Y D5S818:11, 12

Image / Description:   200倍視野下高細胞密度
  100倍視野下高細胞密度
  200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 59: 221-226, 1977; Natl. Cancer Inst. Monogr. 49: 5-9, 1978
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