Cell Line

BCRC Number: 60060
Name: MOLT 4
Type: Human acute lymphoblastic leukemia, T cell
Species: Homo sapiens (human)
Age Stage: 19 years
Gender: Male
Tissue: leukemia; acute lymphoblastic; T lymphoblast

Morphology: Lymphoblast
Split Ratio: Start new cultures at 4 X 10(5) cells/ml
Isoenzyme Analysis: MD; G6PD; LD; NP
Mycoplasma Test: Negative; Note: The line was originally contaminated with mycoplasma but had been cured with antibiotics at ATCC.
Cytogenetics: hypertetraploid; modal chromosome number=95
Karyology: Hypertetraploid; modal number = 95
Tumorigenic: yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate+ 10% fetal bovine serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium; subculture before the cell density reaches 2 x 106 cells/ml
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: 4 x 105 cells/ml
Medium Renewal: every 2 to 3 days

Description: established from cells taken from a patient in relapse; the patient had received prior multidrug chemotherapy; there is a G -> A mutation at codon 248 of the p53 gene; P53 is not expressed; the cells do not produce immunoglobulin or Epstein-Barr virus; MOLT-4 was derived from the same patient as the MOLT-3 cell line
Others: STR-PCR profile — D7S820:8, 10, 11 CSF1PO:11, 12, 13 TH01:6, 8 D13S317:12, 13 D16S539:11, 14 vWA:17, 18 TPOX:8 Amelogenin:X, Y D5S818:12
Product Character: high levels of terminal deoxynucleotidyl transferase (TdT) are produced

Image / Description:   200倍視野下低細胞密度

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 49: 891-895, 1972; J. Natl. Cancer Inst. 65: 715-717, 1980; Pro. Natl. Acad. Sci. USA 87: 75555-75559, 1990; Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993
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