Cell Line



BCRC Number: 60059 Add To Bookmark
Name: HT 1197
Type: Human bladder carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 44 years
Gender: Male
Tissue: urinary bladder; carcinoma

Morphology: Epithelial
Split Ratio: 1:2 to 1:5
Isoenzyme Analysis: G6PD, B; MD; LD
Mycoplasma Test: Negative
Karyology: Hypertriploid
Tumorigenic: yes, in mice and hamsters

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium (Eagle) with Earle's BSS , 2mM L-glutamine, 1.5g/L sodium bicarbonate, 0.1mM non-essential amino acids, and 1.0mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from a grade 4, transitional cell carcinoma of the bladder of a 44-year-old Caucasian male who had not received chemotherapy or radiation therapy; the cells grow in soft agar, produce fibrolytic activity and are tumorigenic in mice and hamsters.
Others: STR-PCR profile — D7S820:11, 12 CSF1PO:11, 12 TH01:6, 9.3 D13S317:11, 12 D16S539:12, 13 vWA:16, 18 TPOX:11, 12 Amelogenin:X, Y D5S818:12
Product Character: fibrinolytic activity

Image / Description:   No Description.
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Biosafety Level: 1
Reference: J. Natl. Cancer. Inst. 58: 881-890, 1977
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