Cell Line



BCRC Number: 60058 Add To Bookmark
Name: HT 1376
Type: Human bladder carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 58 years; grade 3
Gender: Female
Tissue: urinary bladder; carcinoma

Morphology: Epithelial
Split Ratio: 1:2 to 1:6
Isoenzyme Analysis: NP; G6PD, B; MD; LD
Mycoplasma Test: Negative
Karyology: Hypertetraploid; modal number = 111
Receptor expressed: yes, in mice and hamsters

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle’s BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from a grade 3, transitional cell carcinoma of the bladder of a 58-year-old Caucasian woman who had not received chemotherapy or radiation; the tumor was invasive and moderately pleomorphic; the line does not contain detectable virus particles, herpesvirus antigens or retrovirus antigens
Others: STR-PCR profile — D7S820:9, 12 CSF1PO:12 TH01:7, 10 D13S317:9, 11 D16S539:11, 14 vWA:15, 18 TPOX:8 Amelogenin:X D5S818:11, 12
Product Character: fibrinolytic activity; interferon

Image / Description:   200倍視野下高細胞密度
  200倍視野下低細胞密度
  100倍視野下高細胞密度
  200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 58: 881-890, 1977; Arch. Virol. 62: 209-219, 1979
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