Cell Line



BCRC Number: 60057 Add To Bookmark
Name: PK(15)
Type: Porcine kidney
Species: Sus scrofa (pig) Synonym(s): swine
Age Stage: Adult
Gender: unknown
Tissue: Kidney; normal

Morphology: Epithelial
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: MD; G6PD; LD
Mycoplasma Test: negative
Virus resistance: poliovirus 2
Virus susceptibility: hog cholera; African swine fever; vesicular exanthema of swine; foot and mouth disease (FMDV); vesicular stomatitis (Indiana); vaccinia; reovirus 2, 3; adenovirus 4, 5; coxsackievirus B2, B3, B4, B5, B6

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 95% Minimum essential medium (Eagle) with Earle's BSS , 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1mM non-essential amino acids, and 1.0 mM sodium pyruvate + 5% bovine calf serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: The cell line harbors an endogenous C-type retrovirus; the cells are positive for porcine circovirus (PCV) antigens; the cells are positive for keratin by immunoperoxidase staining.
Product Character: plasminogen activator; keratin

Image / Description:   200倍視野下高細胞密度
  200倍視野下高細胞密度
  100倍視野下高細胞密度
 

Biosafety Level: 1
Reference: Can. J. Vet. Res. 53: 431-433, 1989; Am. J. Vet. Res. 29: 153-164, 1968; J. Gen. Virol. 10: 195-198, 1971
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