Cell Line



BCRC Number: 60056
Name: ZR-75-30
Type: Human breast carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Black
Age Stage: 47 years
Gender: Female
Tissue: Breast; mammary gland; ductal carcinoma; from metastatic site: ascites

Morphology: Epithelial
Split Ratio: 1:2 to 1:3
Isoenzyme Analysis: MD; G6PD; LD
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90%RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate+ 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Others: STR-PCR profile — D7S820:11, 12 CSF1PO:10, 11 TH01:8, 9 D13S317:10, 11 D16S539:9 vWA:18, 19 TPOX:10, 11 Amelogenin:X D5S818:12, 13

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Biosafety Level: 1
Reference: Cancer Res. 38: 3352-3364, 1978
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