Cell Line



BCRC Number: 60055
Name: ZR-75-1
Type: Human breast carcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 63 years
Gender: Female
Tissue: Breast; mammary gland; ductal carcinoma; from metastatic site: ascites

Morphology: Epithelial
Split Ratio: 1:4 to 1:6
Isoenzyme Analysis: MD, G6PD, LD
Mycoplasma Test: Negative
Cytogenetics: hypertriploid
Karyology: Hypertriploid; modal chromosome number = 72
Tumorigenic: yes, forms tumors in nude mice
Receptor expressed: estrogen receptor, positive

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate+ 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from a malignant ascitic effusion in a 63-year-old Caucasian female with infiltrating ductal carcinoma; the cells possess receptors for estrogen and other steroid hormones; they produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.
Others: STR-PCR profile — D7S820:10, 11 CSF1PO:10, 11 TH01:7, 9.3 D13S317:9 D16S539:11 vWA:16, 18 TPOX:8 Amelogenin:X D5S818:13
Product Character: mucin (apomucin, MUC-1, MUC-2)

Image / Description:   No Description.
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Biosafety Level: 1
Reference: Cancer Res. 38: 3352-3364, 1978; Cancer Res. 38: 4327-4339, 1978; Cancer Res. 53: 1437-1443, 1993
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