Cell Line

BCRC Number: 60054
Name: COLO 205
Type: Human colon adenocarcinoma
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 70 years
Gender: Male
Tissue: Colon; adenocarcinoma; from metastatic site: ascites

Morphology: mixed, adherent and suspension.
Split Ratio: 1:2 to 1:6
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Cytogenetics: hypertriploid
Karyology: Hypertriploid; 10-11 marker chromosomes were common
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate+ 10% heat-inactivated fetal bovine serum
Growth Property: mixed, adherent and suspension, grow loosely attached and in suspension
SubCulture Procedure: collect floating cells by centrifugation; use trypsin-EDTA for attached cells
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Isolated from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon; the patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen; the cells are CSAp negative (CSAp-); the cells are positive for keratin by immunoperoxidase staining.
Others: STR-PCR profile — D7S820:9,10 CSF1PO:11, 12 TH01:8, 9 D13S317:10, 12 D16S539:12, 13 vWA:15 TPOX:11 Amelogenin:X D5S818:10, 13
Product Character: carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10)

Image / Description:   100倍視野下低細胞密度

Biosafety Level: 1
Reference: Cancer Res. 38: 1345-1355, 1978; Int. J. Cancer 41: 287-296, 1988; Int. J. Cancer 55: 96-101, 1993; J. Biol. Chem. 268: 24232-24241, 1993
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