Cell Line



BCRC Number: 60053
Name: LS 174T
Type: Human colorectal adenocarcinoma, Dukes' type B
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: 58 years; Dukes' type B
Gender: Female
Tissue: Colon; adenocarcinoma

Morphology: Epithelial
Split Ratio: 1:2 to 1:5
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Cytogenetics: 45,X; one X chromosome missing; no other chromosomal aberrations
Karyology: 45, X with missing one X chromosome
Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium (Eagle) with Earle's BSS , 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: this is a trypsinized variant of LS 180 (see ATCC CL-187, or CCRC 60100) colon adenocarcinoma line; it is more easily subcultivated than that parent line and produce large amounts of carcinoembryonic antigen (CEA); the cell line is positive for expression of c-myc, N-myc, H-ras, N-ras. Myb, and fos oncogenes. K-ras and sis oncogene expression were not detected.
Others: STR-PCR profile — D7S820:11 CSF1PO:10, 14 TH01:6, 7 D13S317:10 D16S539:11, 13 vWA:15, 17 TPOX:8, 9 Amelogenin:X D5S818:11, 15
Product Character: carcinoembryonic antigen (CEA) 1944 ng/10 exp6 cells in 10 days; mucin; interleukin 10 (IL-10, interleukin-10); interleukin 6 (IL-6, interleukin-6)

Image / Description:   200倍視野下高細胞密度
 

Biosafety Level: 1
Patent Statement: U.S. Patent 4,228,236
Reference: In Vitro 12: 180-191, 1976; U.S. Pat. 4,228,236 dated Oct. 14, 1980; Int. J. Cancer 41: 287-296, 1988; Cancer Genet. Cytogenet. 10: 351, 1983; Int. J. Cancer 55: 96-101, 1993
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