Cell Line



BCRC Number: 60052
Name: P19
Type: Mouse teratocarcinoma
Species: Mus musculus (mouse)
Strain Ethnicity: C3H/He
Gender: Male
Tissue: embryo; teratocarcinoma; embryonal carcinoma

Morphology: epithelial
Split Ratio: 1:10
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Euploid; stemline number = 40

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Alpha minimum essential medium with ribonucleosides and deoxyribonucleosides+ 2.5% fetal bovine serum + 7.5% calf serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA; NOTE: Do not allow the cells to become confluent.
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: Add fresh medium at least every 48 hours

Description: Derived from an embryonal carcinoma induced in a C3H/He strain mouse; the line can be cloned at high efficiency in medium containing 0.1mM 2-mercaptoethanol; the cells are pluripotential; the cells can be induced to differentiate into neural and glial-like cel.

Image / Description:   200倍視野下高細胞密度
  200倍視野下低細胞密度
 

Biosafety Level: 1
Reference: Dev. Biol. 89: 503-508, 1982; J. Cell Biol. 94: 253-262, 1982; Nature 299: 165-167, 1982
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