Cell Line



BCRC Number: 60051
Name: Hepa 1-6
Type: Mouse hepatoma
Species: Mus musculus (mouse)
Strain Ethnicity: C57/L
Gender: unknown
Tissue: Liver; hepatoma

Morphology: Epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: MD, G6PD, LD
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose+ 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Product Character: albumin, alpha fetoprotein (AFP, alpha-fetoprotein); albumin; alpha 1 antitrypsin (alpha-1-antitrypsin); amylase

Image / Description:   200倍視野下高細胞密度
  200倍視野下低細胞密度
  100倍視野下高細胞密度
  200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 64: 809-819, 1980; Methods Enzymol. 151: 19-38, 1987
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