Cell Line



BCRC Number: 60050
Name: LL/2 (LLC1)
Type: Mouse Lewis lung carcinoma
Species: Mus musculus (mouse)
Strain Ethnicity: C57BL
Gender: unknown
Tissue: Lung; Lewis lung carcinoma

Morphology: rounded - loosely attached or floating
Split Ratio: 1:4 to 1:6
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Tumorigenic: yes, in C57BL mice
Doubling time: 21 hours

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: mixed: adherent and suspension; the cells form multilayers in flasks without actually reaching confluency
SubCulture Procedure: Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with trypsin-EDTA solution
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma; the cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate; the cells are reported to be highly tumorigenic, but weakly metastatic in mice.

Image / Description:   200倍視野下低細胞密度
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Biosafety Level: 1
Reference: Cancer Lett. 11: 63-73, 1980
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