Cell Line

BCRC Number: 60049
Name: L 1210
Type: Mouse lymphocytic leukemia
Species: Mus musculus (mouse)
Strain Ethnicity: DBA subline 212
Age Stage: 8 months
Gender: Female
Tissue: lymphocytic leukemia

Morphology: Lymphoblast
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Diploid; modal number = 40
Tumorigenic: yes, in nude mice
Doubling time: 8 to 10 hrs

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4.5 g/L glucose + 10% horse serum
Growth Property: Suspension
SubCulture Procedure: Addition or replacement of fresh medium
Freezed Medium: 93% culture medium + 7% DMSO
Seeding Density: Start cultures at 5 X 10 exp4 viable cells/ml,4-6 x 104 cells ml
Medium Renewal: every 2 to 3 days

Description: established from a tumor in female #234 that developed following skin paintings with 0.2% methylcholanthrene in ether; the line has been used extensively for routine screening programs of chemical agents and natural products for cytotoxic activity; it is also utilized in preliminary testing for antitumor activity by the NCI in cancer chemotherapy screening studies.

Image / Description:   200倍視野下高細胞密度

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 10: 179-192, 1949; J. Natl. Cancer Inst. 36: 405-421, 1966; Cancer Chemother. Rep. 51: 451-453, 1967
Frozen Vial for all NTD$ 6000 Order this item

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