Cell Line

BCRC Number: 60048 Add To Bookmark
Name: PC-12
Type: Rat adrenal pheochromocytoma
Species: Rattus norvegicus (rat)
Gender: Male
Tissue: Adrenal gland; pheochromocytoma

Morphology: polygonal, suspension
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Tumorigenic: yes, in New England Deaconess Hospital strain rats
Receptor expressed: nerve growth factor (NGF)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 85% RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate + 10% heat-inactivated horse serum + 5% fetal bovine serum
Growth Property: small clusters, poorly adherent
SubCulture Procedure: Remove cells from substrate by pipetting over the cell sheet with fresh medium, by striking flask or by scraping. Aspirate cells in fresh medium with a small bore pipette to break up clusters (or pass the suspension several times through a 22 gauge needle
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Established from a transplantable rat adrenal pheochromocytoma; the cells respond reversibly to NGF (nerve growth factor) by induction of the neuronal phenotype; the cells synthesize and store the catecholamine neurotransmitters dopamine and norepinephrin.
Product Character: catecholamines; dopamine; norepinephrine

Image / Description:   100倍視野下低細胞密度

Biosafety Level: 1
Reference: Proc. Natl. Acad. Sci. USA 73: 2424-2426, 1976; Science 229: 393-395, 1985; EMBO 2: 643-648, 1983
growing cell in 15ml tube for all NTD$ 6000 Order this item

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