Cell Line



BCRC Number: 60044 Add To Bookmark
Name: BCE C/D-1b
Type: bovine cornea endothelial
Species: Bos taurus (bovine)
Age Stage: adult
Gender: Male
Tissue: eye; cornea; endothelial

Morphology: endothelial
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: NP, G6PD, MD, LD
Mycoplasma Test: negative
Doubling time: The reported population doubling time decreases with increasing passages from approximately 55 hours at passage 70 to 30 hours at passage 90.

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: established from explants of normal adult bovine corneas; the cells produce a extracellular matrix that can be used to support the growth of other cells including rat neuronal cells and astrocytes; the cells will grow well (but at a slower rate) in medium containing decreased amounts of serum (5% bovine calf serum, 2.5% fetal bovine serum).
Product Character: extracellular matrix (ECM) proteins

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Biosafety Level: 1
Reference: Exp. Eye Res. 25: 75-89, 1977; Mol. Cell. Endocrinol. 37: 319-327, 1984
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