Cell Line

BCRC Number: 60041 Add To Bookmark
Name: BHK-21 (C-13)
Type: Syrian hamster kidney
Species: Mesocricetus auratus (hamster, Syrian golden)
Age Stage: 1 day old newborn
Gender: unknown
Tissue: Kidney; normal

Morphology: Fibroblast
Split Ratio: 1:2 to 1:10
Isoenzyme Analysis: NP; LD; G6PD; MD; MPI; Pep B; AST
Mycoplasma Test: Negative
Virus resistance: poliovirus 2
Virus susceptibility: adenovirus 25; herpes simplex; reovirus 3; vesicular stomatitis (Indiana)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: The parent line of BHK-21(C-13) was derived from the kidneys of five unsexed, 1-day-old hamsters; following 84 days of continuous cultivation, interrupted only by an 8-day preservation by freezing, clone 13 was initiated by single-cell isolation; the line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII)

Image / Description:   200倍視野下低細胞密度

Biosafety Level: 1
Reference: Virology 16: 147-151, 1962; J. Natl. Cancer Inst. 30: 795-815, 1963
Frozen Vial for all NTD$ 6000 Order this item

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