Cell Line

BCRC Number: 60039
Name: A 375
Type: Human malignant melanoma
Species: Homo sapiens (human)
Age Stage: 54 years
Gender: Female
Tissue: melanoma; malignant melanoma; skin

Morphology: Epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Hypotriploid; modal number = 62
Tumorigenic: yes, in immunosuppressed mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from a 54-year-old female with malignant melanoma; the line produced rapidly growing subcutaneous tumors resembling amelanotic melanomas in antithymocyte, serum-treated NIH Swiss mice and formed colonies on normal fibroblast and in agar.
Others: STR-PCR profile — D7S820:9 CSF1PO:11, 12 TH01:8 D13S317:11,14 D16S539:9 vWA:16, 17 TPOX:8, 10 Amelogenin:X D5S818:12

Image / Description:   200倍視野下高細胞密度

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 51: 1417-1423, 1973
Frozen Vial for all NTD$ 6000 Order this item

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

web maintainance: +886-3-5223191 ext 593

Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).