Cell Line

BCRC Number: 60039 Add To Bookmark
Name: A 375
Type: Human malignant melanoma
Species: Homo sapiens (human)
Age Stage: 54 years
Gender: Female
Tissue: melanoma; malignant melanoma; skin

Morphology: Epithelial
Split Ratio: 1:3 to 1:8
Isoenzyme Analysis: NP; G6PD; MD; LD
Mycoplasma Test: Negative
Karyology: Hypotriploid; modal number = 62
Tumorigenic: yes, in immunosuppressed mice

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Derived from a 54-year-old female with malignant melanoma; the line produced rapidly growing subcutaneous tumors resembling amelanotic melanomas in antithymocyte, serum-treated NIH Swiss mice and formed colonies on normal fibroblast and in agar.
Others: STR-PCR profile — D7S820:9 CSF1PO:11, 12 TH01:8 D13S317:11,14 D16S539:9 vWA:16, 17 TPOX:8, 10 Amelogenin:X D5S818:12

Image / Description:   200倍視野下高細胞密度

Biosafety Level: 1
Reference: J. Natl. Cancer Inst. 51: 1417-1423, 1973
Frozen Vial for all NTD$ 6000 Order this item

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