Cell Line



BCRC Number: 60038 Add To Bookmark
Name: Hs68
Type: Human foreskin fibroblast
Species: Homo sapiens (human)
Strain Ethnicity: Caucasian
Age Stage: Newborn
Gender: Male
Tissue: skin; foreskin; fibroblast; aspartoacylase deficiency; possible Canavan disease

Morphology: Fibroblast
Split Ratio: 1:4 to 1:8
Isoenzyme Analysis: NP, G6PD, MD, NP, LD
Mycoplasma Test: Negative
Cytogenetics: diploid; normal 46,XY

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 90% Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose + 10% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: obtained from an apparently normal Caucasian newborn male; the cells could be propagated for 42 passages.
Others: STR-PCR profile — D7S820:11, 12 CSF1PO:9, 12 TH01:6, 9.3 D13S317:11, 13 D16S539:11, 12 vWA:16 TPOX:11 Amelogenin:X, Y D5S818:8, 11
Product Character: A GFP-expressing variant of this cell line is also available (BCRC 60644, Hs68-GFP).

Image / Description:   200倍視野下低細胞密度
  200倍視野下高細胞密度
  100倍視野下高細胞密度
  200倍視野下高細胞密度
  100X視野下高細胞密度
  200X視野下高細胞密度
 

Biosafety Level: 1
Reference: Journal of Neurochemistry 55: 129-135, 1991
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